MIALAB

PPIformer Server

About PPIformer

PPIformer server is used to predict interface residue pairs of pentamer protein and maintained by MIALAB at Renmin University of China.

Submitting Jobs

  • Input Requirements: Five FASTA files as input
  • File Format: FASTA format files only
  • File Naming: "proteinname_chainname.fasta" (e.g., "1a4y_A.fasta")
  • Multiple Files: Each file with only one chain

Job Output

  • Content: Interaction prediction between residue pairs
  • Format: Binary result (1=interaction, 0=no interaction)
  • Target: Pentamer protein interface prediction

Research Paper

PPIformer: End-to-End Transformer-Based Siamese Network with attention-based BiGRU to Predict Interface Residue Pairs of Pentamer Protein Based on Sequence Only

Biochimica et Biophysica Acta (BBA) - Proteins and Proteomics. 2020. 1868(11):14054. doi: 10.1016/j.bbapap.2020.140504. Download Paper

First Usage Guide

  • 1
    Upload data files - Select your FASTA files using the upload button
  • 2
    Click Submit button - Process your uploaded files
  • 3
    Click Run button - Execute the prediction and wait a few minutes
  • 4
    Click Send button - You will receive an email about the results
  • 5
    Click Reset button - Clear the form for new analysis

If you have any questions during operation, contact us through xinqigong@ruc.edu.cn

Download Example

Download example FASTA files to test the server

Example Files

Source Code

Get the complete source code and documentation

GitHub Repository
Time Reminder: Predicting protein 3x7 (3x7 single chain residues number is 100, a total of five chains) to get 10 files takes approximately 1.5 minutes. Please wait patiently for the results.

Submit FASTA Files

Upload your pentamer protein sequences for interface prediction

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Please select files before submitting.
Select 5 FASTA files (one chain per file)
Optional: Results will be sent to this email address
Reset Form

Upload Status

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Run Analysis

Execute PPIformer interface prediction on uploaded files

Reset

Analysis Results